Hatamoto, M., Nakai, K., Ohashi, A. & Imachi, H. (2009). Sequence specific bacterial growth inhibition by peptide nucleic acid targeted to the mRNA inding site of 16S rRNA. Appl. Microbiol. Biotechnol. in press.

Sakai, S., Imachi, H., Sekiguchi, Y., Tseng, I-C., Ohashi, A. Harada, H. & Kamagata, Y. (2009). Cultivation of methanogens under low hydrogen conditions by using the coculture method. Appl. Environ. Microbiol. in press.

Miyashita, A., Mochimaru, H. Kazama, H. Ohashi, A. Yamaguchi, T. Nunoura, T. Horikoshi, K. Takai, K. & Imachi, H. (2009). Development of 16S rRNA gene-targeted primers for detection of archaeal anaerobic methanotrophs (ANMEs). FEMS Microbiol. Lett. in press.

Iguchi, A., Terada, T., Narihiro, T., Yamaguchi, T., Kamagata Y. & Sekiguchi, Y. (2009). In Situ Detection and Quantification of Uncultured Members of the Phylum Nitrospirae Abundant in Methanogenic Wastewater Treatment Systems. Microbes and Environments, 24(2), 97-104.

Narihiro, T., Terada, T., Kikuchi, K., Iguchi, A., Ikeda, M., Yamauchi, T., Shiraishi, K., Kamagata Y. Nakamura, K. & Sekiguchi, Y. (2009). Comparative Analysis of Bacterial and Archaeal Communities in Methanogenic Sludge Granules from Upflow Anaerobic Sludge Blanket Reactors Treating Various Food-Processing, High-Strength Organic Wastewaters. Microbes and Environments, 24(2), 88-96.

Imachi, H., S. Sakai, H. Nagai, T. Yamaguchi & K. Takai. (2009). Methanofollis ethanolicus sp. nov., an ethanol-utilizing methanogen isolated from a lotus field. Int. J. Syst. Evol. Microbiol. 59, 800-805.

Yoochatchaval, W., Tsushima, I., Yamaguchi, T., Araki, N., Sumino, H., Ohashi, A., Harada, H. & Syutsubo, K. (2009). Influence of sugar content of wastewater on the microbial characteristics of granular sludge developed at 20°C in the anaerobic granular sludge bed reactor, Journal of Environmental Science and Health, PART A (Toxic/Hazardous Substance & Environmental Engineering), 44, 921–927.


Hui-Ping Chuang, Takashi Yamaguchi, Hideki Harada and Akiyoshi Ohashi (2008). Anoxic Ammonium Oxidation by Application of a Down-Flow Hanging Sponge (DHS) Reactor, J. Environ. Eng. Manage., 18(6), 409-417.

Yoochatchaval, W., Tsushima, I., Ohashi, A., Harada, H., Yamaguchi, T., Araki, N. and Syutsubo, K.(2008) Changes in process performance and microbial characteristics of retained sludge during low-temperature operation of an EGSB reactor, Journal of Environmental Science and Health, Vol. A43, No.14, 1650-1656.

Yoochatchaval, W., Ohashi, A., Harada, H., Yamaguchi, T. and Syutsubo, K. (2008). Intermittent effluent recirculation for the efficient treatment of low strength wastewater by an EGSB, International Journal of Environmental Research, 2(3), 231-238.

S. Uemura, A. Ohashi, H. Harada, T. Hoaki, T. Tomozawa, T. Ohara, R. Ojima and T. Ishida (2008), Production of biologically safe digested manure for land application by a full-scale biogas plant with heat-inactication. Waste Management Res., 26: 256-260.

Y. Sekiguchi, M. Muramatsu, H. Imachi, T. Narihiro, A. Ohashi, H. Harada, S. Hanada and Y. Kamagata (2008), Thermodesulfovibrio aggregans and Thermodesulfovibrio thiophilus sp. nov., two anaerobic, thermophilic, sulfate-reducing bacteria isolated from thermophilic methanogenic sludge, and emended description of the genus Thermodesulfovibrio. Int. J. Syst. Evol. Microbiol. 58: 2541-2548.

H. Imachi, S. Sakai, H. Hirayama, S. Nakagawa, T. Nunoura, K. Takai and K. Horikoshi (2008), Exilispira thermophila gen. nov., sp. nov., an anaerobic, thermophilic spirochete isolated from a deep-sea hydrothermal vent chimney. Int. J. Syst. Evol. Microbiol. 58: 2258-2265.

M. Hatamoto, H. Imachi, Y. Yashiro, A. Ohashi, and H. Harada (2008), Detection of Active Butyrate-Degrading Microorganisms in Methanogenic Sludges by RNA-Based Stable Isotope Probing. Appl. Envir. Microbiol., 74: 3610-3614.

T. Nunoura, H. Oida, J. Miyazaki, A. Miyashita H. Imachi and K. Takai (2008), Quantification of mcrA by fluorescent PCR in methanogenic and anaerobic methanotrophic microbial communities. FEMS Microbiol. Ecol. 64:240-247.

S. Sakai, H., Imachi, S., Hanada, A. Ohashi, H. Harada. and Y. Kamagata (2008), Methanocella paludicola gen. nov., sp. nov., a methane producing archaeon that is the first isolate of the lineage ‘Rice Cluster I’ , and proposal of the new archaeal order Methanocelladales ord. nov., Int J Syst Evol Microbiol 2008 58: 929-936.

K. Kubota, H. Imachi, S. Kawakami, K. Nakamura, H. Harada and A. Ohashi (2008), Evaluation of enzymatic cell treatments for application of CARD-FISH to methanogens. J. Microbiol. Methods. 72: 54-59.

H. Imachi, S. Sakai, Y. Sekiguchi, A. Ohashi, H. Harada, S. Hanada and Y. Kamagata (2008), Methanolinea tarda gen. nov. sp. nov., a methane-producing archaeon isolated from a methanogenic digester sludge.Int. J. Syst. Evol. Microbiol. 58: 294-301.


H.-P. Chuang, H. Imachi, M. Tandukar, S. Kawakami, H. Harada and A. Ohashi (2007), Microbial community that catalyzes partial nitrification at low oxygenatmosphere as revealed by 16S rRNA and amoA genes. J. Bioeng. Biosci., in press

T. Yamada, H. Imachi, A. Ohashi, H. Harada, S. Hanada, Y. Kamagata and Y. Sekiguchi (2007), Bellilinea caldifistulae gen. nov., sp. nov. and Longilineaarvoryzae gen. nov., sp. nov., strictly anaerobic, filamentous bacteria ofthe phylum Chloroflexi isolated from methanogenic propionate-degradingconsortia. Int. J. Syst. Evol. Microbiol., 57: 2299-2306.

H. Imachi, S. Sakai, A. Ohashi, H. Harada, S. Hanada, Y. Kamagata and Y. Sekiguchi (2007), Pelotomaculum propionicicum sp. nov., an anaerobic, mesophilic, obligately syntrophic propionate-oxidizing bacterium. Int. J. Syst. Evol. Microbiol., 57: 1487-1492.

S. Sakai, H. Imachi, Y. Sekiguchi, A. Ohashi, H. Harada, Y. Kamagata (2007), Isolation of Key Methanogens for Global Methane Emission from Rice Paddy Field: a Novel Isolate Affiliated with a Clone Cluster, the Rice Cluster I. Appl. Envir. Microbiol., 73: 4326-4331.

M. Hatamoto, H. Imachi, Y. Yashiro, A. Ohashi, and H. Harada (2007), Diversity of Anaerobic Microorganisms involved in long-chain fatty acid degradation in methanogenic sludges as revealed by RNA-based stable isotope probing Appl. Environ. Microbiol., 73: 4119-4127.

M. Hatamoto, H. Imachi, S. Fukayo, A. Ohashi, and H. Harada (2007), Syntrophomonas palmitatica sp. nov., an anaerobic, syntrophic, long-chain fatty-acid-oxidizing bacterium isolated from methanogenic sludge. Int. J. Syst. Evol. Microbiol., 57: 2137-2142.

M. Hatamoto, H. Imachi, A. Ohashi, and H. Harada (2007), Identification and cultivation of anaerobic, syntrophic long-chain fatty acid-degrading microbes from mesophilic and thermophilic methanogenic sludges. Appl. Environ. Microbiol., 73:1332-1340.

M. Tandukar, A. Ohashi and H. Harada (2007), Performance comparison of a pilot-scale UASB and DHS system and activated sludge process for the treatment of municipal wastewater, Water Research, Volume 41, Issue 12, pp. 2697-2705.

H.-P. Chuang, A. Ohashi, H. Imachi, T. Madan and H. Harada (2007), Effective partial nitrification to nitrite by down-flow hanging sponge reactor under limited oxygen condition. Water Research, Vol.41 Issue 2, pp.295-302.

N. Sato, T. Okubo, T. Onodera, Lalit K. Agrawal, A. Ohashi, H. Harada (2007), Economic evaluation of sewage treatment processes in India. J. Environmental Management, Vol. 84, pp. 447-460.


T. Yamada, Y. Sekiguchi, S. Hanada, H. Imachi, A. Ohashi, H. Harada and Y. Kamagata (2006), Anaerolinea thermolimosa sp. nov., Levilinea saccharolytica gen. nov., sp. nov. and Leptolinea tardivitalis gen. nov., sp. nov., novel filamentous anaerobes, and description of the new classes Anaerolineae classis nov. and Caldilineae classis nov. in the bacterial phylum Chloroflexi. Int J Syst Evol Microbiol, 56, 1331 – 1340.

Y. Sekiguchi, H. Imachi, A. Susilorukmi, M. Muramatsu, A. Ohashi, H. Harada and Y. Kamagata (2006), Tepidanaerobacter syntrophicus gen. nov., sp. nov., an anaerobic, moderately thermophilic, syntrophic alcohol- and lactate-degrading bacterium isolated from thermophilic digested sludges. Int. J. Syst. Evol. Microbiol. 56, 1621-1629.

H. Imachi, Y. Sekiguchi, Y. Kamagata, A. Loy, Y.-L. Qiu, P. Hugenholtz, N. Kimura, M. Wagner, A. Ohashi,and H. Harada (2006), Non-Sulfate-Reducing, Syntrophic Bacteria Affiliated with Desulfotomaculum Cluster I Are Widely Distributed in Methanogenic Environments. Appl. Environ. Microbiol. 72: 2080-2091. (IF=3.818)

K. Kubota, A. Ohashi, H. Imachi, and H. Harada (2006), Improved in situ hybridization efficiency with locked-nucleic-acid-incorporated DNA probes. Appl. Environ. Microbiol. Vol.72, pp.5311-5317. (IF=3.818)

K. Kubota, A. Ohashi, H. Imachi, and H. Harada (2006), Visualization of mcr mRNA in a methanogen by fluorescence in situ hybridization with an oligonucleotide probe and two-pass tyramide signal amplification (two-pass TSA-FISH), J. Microbiol. Methods. Vol.66, pp.521-528. (IF=2.015)

M. Tandukar, S. Uemura, A. Ohashi, H. Harada (2006), Combining UASB and the ‘Fourth Generation’ DHS (Down-Flow Hanging Sponge) Reactor for Municipal Wastewater Treatment. Water Sci. and Tech., vol.53, No.31, pp209-218.

M. Yamada, M. Yamauchi, T. Suzuki, A. Ohashi and H. Harada (2006), On-site treatment of high-strength alcohol distillery wastewater by a pilot-scale thermophilic multi-staged UASB (MS-UASB) reactor, Water Sci. & Tech., Vol.53, No.3, pp27-35.

M. Tandukar, I Machdar, S. Uemura, A Ohashi, and H Harada (2006), “Potential of a Combination of UASB and DHS (Down-flow Hanging Sponge) Reactor as a Novel Sewage Treatment System for Developing Countries”, J. of Environ. Eng., (American Society of Civil Engineers), vol.132, No.2, pp166-172

N. Sato, T. Okubo, T. Onodera, A. Ohashi, H. Harada (2006) “Prospects for Self-sustainable Sewage Treatment System: A Case Study on Full-scale UASB System in India’s Yamuna River Basin”, J. Environmental Management, Vol.80, pp.198-207.

T. Yamaguchi, Y. Bungo, M. Takahashi, H. Sumino, A. Nagano, N. Araki, T. Imai, S. Yamazaki, H. Harada (2006), Low strength wastewater treatment under low temperature conditions by a novel sulfur redox action process., Water Sci. & Tech., Vol.53, No.6, pp99-105.

N. Araki, Y. Tsukamoto, A. Nagano, T. Yamaguchi, H. Harada (2006), Real-time PCR quantification of nitrite reductase (nirS) genes in a nitrogen removing fluidized bed reactor., Water Sci. & Tech., Vol.53, No.6, pp59-65.

T. Tokutomi, T. Kiyokawa, C. Shibayama, H. Harada, A. Ohashi (2006), Effect of inorganic carbon on nitrite accumulation in an aerobic granule reactor., Water Sci. & Tech., Vol.53, No.12, pp285-294.

T. Kosaka, T. Uchiyama, S. Ishii, M. Enoki, H. Imachi, Y. Kamagata, A. Ohashi, H. Harada, H. Ikenaga and K. Watanabe (2006), Reconstruction and Regulation of the Central Catabolic Pathway in the Thermophilic Propionate-Oxidizing Syntroph Pelotomaculum thermopropionicum, J. Bacteriol., vol.188, No.1, pp202-210

Qiu, Y.-L. Y. Sekiguchi, S. Hanada, H. Imachi, I-C. Tseng, S.-S. Cheng, A. Ohashi, H. Harada and Y. Kamagata (2006) Pelotomaculum terephthalicum sp. nov. and Pelotomaculum isophthalicum sp. nov.: two anaerobic bacteria that degrade phthalate isomers in syntrophic association with hydrogenotrophic methanogens, Archives of Microbiology

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