Recently, the rRNA approach is widely used for revealing a microbial community and opening the unknown microbial world. In the rRNA approach, fluorescence in situ hybridization (FISH) makes it possible to detect the target cells at single cell resolution. In addition, combination of FISH with single cell technologies, such as cell collection using flow cytometry, whole genome amplification from single cell and so on, become an powerful tools for revealing unknown microbial ecology. Thus, FISH methods have been attracted in environmental microbiology.
Although FISH is a widely used method, there are some problems. For example, cell wall treatment is required to penetrate probes or some reporters, especially for highly sensitive FISH methods, which use high molecular weight substance, such as in situ PCR, CARD-FISH, RING-FISH and so on. Many researchers try to overcome the problems, however, it hasn’t been solved yet. In this study, we are developing new FISH technique, which don’t require a cell wall treatment.