Hui-Ping Chuang, Takashi Yamaguchi, Hideki Harada and Akiyoshi Ohashi (2008). Anoxic Ammonium Oxidation by Application of a Down-Flow Hanging Sponge (DHS) Reactor, J. Environ. Eng. Manage., 18(6), 409-417.

Yoochatchaval, W., Tsushima, I., Ohashi, A., Harada, H., Yamaguchi, T., Araki, N. and Syutsubo, K.(2008) Changes in process performance and microbial characteristics of retained sludge during low-temperature operation of an EGSB reactor, Journal of Environmental Science and Health, Vol. A43, No.14, 1650-1656.

Yoochatchaval, W., Ohashi, A., Harada, H., Yamaguchi, T. and Syutsubo, K. (2008). Intermittent effluent recirculation for the efficient treatment of low strength wastewater by an EGSB, International Journal of Environmental Research, 2(3), 231-238.

S. Uemura, A. Ohashi, H. Harada, T. Hoaki, T. Tomozawa, T. Ohara, R. Ojima and T. Ishida (2008), Production of biologically safe digested manure for land application by a full-scale biogas plant with heat-inactication. Waste Management Res., 26: 256-260.

Y. Sekiguchi, M. Muramatsu, H. Imachi, T. Narihiro, A. Ohashi, H. Harada, S. Hanada and Y. Kamagata (2008), Thermodesulfovibrio aggregans and Thermodesulfovibrio thiophilus sp. nov., two anaerobic, thermophilic, sulfate-reducing bacteria isolated from thermophilic methanogenic sludge, and emended description of the genus Thermodesulfovibrio. Int. J. Syst. Evol. Microbiol. 58: 2541-2548.

H. Imachi, S. Sakai, H. Hirayama, S. Nakagawa, T. Nunoura, K. Takai and K. Horikoshi (2008), Exilispira thermophila gen. nov., sp. nov., an anaerobic, thermophilic spirochete isolated from a deep-sea hydrothermal vent chimney. Int. J. Syst. Evol. Microbiol. 58: 2258-2265.

M. Hatamoto, H. Imachi, Y. Yashiro, A. Ohashi, and H. Harada (2008), Detection of Active Butyrate-Degrading Microorganisms in Methanogenic Sludges by RNA-Based Stable Isotope Probing. Appl. Envir. Microbiol., 74: 3610-3614.

T. Nunoura, H. Oida, J. Miyazaki, A. Miyashita H. Imachi and K. Takai (2008), Quantification of mcrA by fluorescent PCR in methanogenic and anaerobic methanotrophic microbial communities. FEMS Microbiol. Ecol. 64:240-247.

S. Sakai, H., Imachi, S., Hanada, A. Ohashi, H. Harada. and Y. Kamagata (2008), Methanocella paludicola gen. nov., sp. nov., a methane producing archaeon that is the first isolate of the lineage ‘Rice Cluster I’ , and proposal of the new archaeal order Methanocelladales ord. nov., Int J Syst Evol Microbiol 2008 58: 929-936.

K. Kubota, H. Imachi, S. Kawakami, K. Nakamura, H. Harada and A. Ohashi (2008), Evaluation of enzymatic cell treatments for application of CARD-FISH to methanogens. J. Microbiol. Methods. 72: 54-59.

H. Imachi, S. Sakai, Y. Sekiguchi, A. Ohashi, H. Harada, S. Hanada and Y. Kamagata (2008), Methanolinea tarda gen. nov. sp. nov., a methane-producing archaeon isolated from a methanogenic digester sludge.Int. J. Syst. Evol. Microbiol. 58: 294-301.


小野寺崇、Doni SUGIYANA、Madan TANDUKAR、上村 繁樹、長野 晃弘、山口 隆司、大橋 晶良、原田秀樹(2008)新型汚泥保持担体を適用した第6世代型DHS反応器における下水処理性能、土木学会論文集G、Vol. 64、No. 2、pp.78-87.


井町寛之、幡本将史、原田秀樹、(2008). メタン発酵汚泥からの難培養性微生物の分離・培養 (第1章第2節)、バイオガスの最新技術、pp. 15-23、 シーエムシー出版


H.-P. Chuang, H. Imachi, M. Tandukar, S. Kawakami, H. Harada and A. Ohashi (2007), Microbial community that catalyzes partial nitrification at low oxygenatmosphere as revealed by 16S rRNA and amoA genes. J. Bioeng. Biosci., in press

T. Yamada, H. Imachi, A. Ohashi, H. Harada, S. Hanada, Y. Kamagata and Y. Sekiguchi (2007), Bellilinea caldifistulae gen. nov., sp. nov. and Longilineaarvoryzae gen. nov., sp. nov., strictly anaerobic, filamentous bacteria ofthe phylum Chloroflexi isolated from methanogenic propionate-degradingconsortia. Int. J. Syst. Evol. Microbiol., 57: 2299-2306.

H. Imachi, S. Sakai, A. Ohashi, H. Harada, S. Hanada, Y. Kamagata and Y. Sekiguchi (2007), Pelotomaculum propionicicum sp. nov., an anaerobic, mesophilic, obligately syntrophic propionate-oxidizing bacterium. Int. J. Syst. Evol. Microbiol., 57: 1487-1492.

S. Sakai, H. Imachi, Y. Sekiguchi, A. Ohashi, H. Harada, Y. Kamagata (2007), Isolation of Key Methanogens for Global Methane Emission from Rice Paddy Field: a Novel Isolate Affiliated with a Clone Cluster, the Rice Cluster I. Appl. Envir. Microbiol., 73: 4326-4331.

M. Hatamoto, H. Imachi, Y. Yashiro, A. Ohashi, and H. Harada (2007), Diversity of Anaerobic Microorganisms involved in long-chain fatty acid degradation in methanogenic sludges as revealed by RNA-based stable isotope probing Appl. Environ. Microbiol., 73: 4119-4127.

M. Hatamoto, H. Imachi, S. Fukayo, A. Ohashi, and H. Harada (2007), Syntrophomonas palmitatica sp. nov., an anaerobic, syntrophic, long-chain fatty-acid-oxidizing bacterium isolated from methanogenic sludge. Int. J. Syst. Evol. Microbiol., 57: 2137-2142.

M. Hatamoto, H. Imachi, A. Ohashi, and H. Harada (2007), Identification and cultivation of anaerobic, syntrophic long-chain fatty acid-degrading microbes from mesophilic and thermophilic methanogenic sludges. Appl. Environ. Microbiol., 73:1332-1340.

M. Tandukar, A. Ohashi and H. Harada (2007), Performance comparison of a pilot-scale UASB and DHS system and activated sludge process for the treatment of municipal wastewater, Water Research, Volume 41, Issue 12, pp. 2697-2705.

H.-P. Chuang, A. Ohashi, H. Imachi, T. Madan and H. Harada (2007), Effective partial nitrification to nitrite by down-flow hanging sponge reactor under limited oxygen condition. Water Research, Vol.41 Issue 2, pp.295-302.

N. Sato, T. Okubo, T. Onodera, Lalit K. Agrawal, A. Ohashi, H. Harada (2007), Economic evaluation of sewage treatment processes in India. J. Environmental Management, Vol. 84, pp. 447-460.

阿部憲一、大橋晶良、山口隆司、徳富孝明 (2007)、塩分濃度による亜硝酸型硝化の誘導効果の有無、環境工学論文集、Vol. 44、pp.195-200.

大河原正博、西山桂太、山口隆司、珠坪一晃、井町寛之、原田秀樹、大橋晶良 (2007)、Expanded Granular Sludge Bed (EGSB) リアクターによる実下水処理特性の評価、環境工学論文集、Vol. 44、 pp.579-587.

田村英輔、中村猛利、河島正明、Narin PATTANANUWAT、山口隆司、井町寛之、原田秀樹、大橋晶良 (2007)、過負荷による高温UASBリアクター処理性能の破綻および回復、環境工学論文集、Vol. 44、pp.589-596.

大塚勇輝、橋本尚人、荒木信夫、阿部憲一、川上周司、山口隆司、珠坪一晃 (2007)、apsA mRNAを標的としたFISH法による硫酸塩還元菌群の検出、環境工学論文集、Vol. 44、pp.633-639.

上村繁樹、高橋克夫、高石斌夫、丸山陽佑、鈴木紗織、山口隆司、大橋晶良、原田秀樹 (2007)、下向流スポンジキューブ懸垂型(DHS)リアクターの処理性能に及ぼすスポンジサイズの影響、下水道協会誌、Vol. 44、No. 541、pp. 94-103.

上村繁樹、鈴木紗織、伊藤昌宏、徳富孝明、原田秀樹、井町寛之、阿部憲一、大橋晶良 (2007)、スポンジ-エアリフト型リアクターによる部分的亜硝酸化に及ぼす影響因子、用水と廃水、Vol. 49、No. 9、pp. 56-63.

<総説・解説・著書 >

酒井早苗、井町寛之 (2007)、水田土壌からのメタン生成キープレイヤー-Rice Cluster Iメタン生成古細菌を”嫌気共生培養法”で純粋分離に成功-、化学と生物、第45巻12号、pp. 814-816.


T. Yamada, Y. Sekiguchi, S. Hanada, H. Imachi, A. Ohashi, H. Harada and Y. Kamagata (2006), Anaerolinea thermolimosa sp. nov., Levilinea saccharolytica gen. nov., sp. nov. and Leptolinea tardivitalis gen. nov., sp. nov., novel filamentous anaerobes, and description of the new classes Anaerolineae classis nov. and Caldilineae classis nov. in the bacterial phylum Chloroflexi. Int J Syst Evol Microbiol, 56, 1331 – 1340.

Y. Sekiguchi, H. Imachi, A. Susilorukmi, M. Muramatsu, A. Ohashi, H. Harada and Y. Kamagata (2006), Tepidanaerobacter syntrophicus gen. nov., sp. nov., an anaerobic, moderately thermophilic, syntrophic alcohol- and lactate-degrading bacterium isolated from thermophilic digested sludges. Int. J. Syst. Evol. Microbiol. 56, 1621-1629.

H. Imachi, Y. Sekiguchi, Y. Kamagata, A. Loy, Y.-L. Qiu, P. Hugenholtz, N. Kimura, M. Wagner, A. Ohashi,and H. Harada (2006), Non-Sulfate-Reducing, Syntrophic Bacteria Affiliated with Desulfotomaculum Cluster I Are Widely Distributed in Methanogenic Environments. Appl. Environ. Microbiol. 72: 2080-2091. (IF=3.818)

K. Kubota, A. Ohashi, H. Imachi, and H. Harada (2006), Improved in situ hybridization efficiency with locked-nucleic-acid-incorporated DNA probes. Appl. Environ. Microbiol. Vol.72, pp.5311-5317. (IF=3.818)

K. Kubota, A. Ohashi, H. Imachi, and H. Harada (2006), Visualization of mcr mRNA in a methanogen by fluorescence in situ hybridization with an oligonucleotide probe and two-pass tyramide signal amplification (two-pass TSA-FISH), J. Microbiol. Methods. Vol.66, pp.521-528. (IF=2.015)

M. Tandukar, S. Uemura, A. Ohashi, H. Harada (2006), Combining UASB and the ‘Fourth Generation’ DHS (Down-Flow Hanging Sponge) Reactor for Municipal Wastewater Treatment. Water Sci. and Tech., vol.53, No.31, pp209-218.

M. Yamada, M. Yamauchi, T. Suzuki, A. Ohashi and H. Harada (2006), On-site treatment of high-strength alcohol distillery wastewater by a pilot-scale thermophilic multi-staged UASB (MS-UASB) reactor, Water Sci. & Tech., Vol.53, No.3, pp27-35.

M. Tandukar, I Machdar, S. Uemura, A Ohashi, and H Harada (2006), “Potential of a Combination of UASB and DHS (Down-flow Hanging Sponge) Reactor as a Novel Sewage Treatment System for Developing Countries”, J. of Environ. Eng., (American Society of Civil Engineers), vol.132, No.2, pp166-172

N. Sato, T. Okubo, T. Onodera, A. Ohashi, H. Harada (2006) “Prospects for Self-sustainable Sewage Treatment System: A Case Study on Full-scale UASB System in India’s Yamuna River Basin”, J. Environmental Management, Vol.80, pp.198-207.

T. Yamaguchi, Y. Bungo, M. Takahashi, H. Sumino, A. Nagano, N. Araki, T. Imai, S. Yamazaki, H. Harada (2006), Low strength wastewater treatment under low temperature conditions by a novel sulfur redox action process., Water Sci. & Tech., Vol.53, No.6, pp99-105.

N. Araki, Y. Tsukamoto, A. Nagano, T. Yamaguchi, H. Harada (2006), Real-time PCR quantification of nitrite reductase (nirS) genes in a nitrogen removing fluidized bed reactor., Water Sci. & Tech., Vol.53, No.6, pp59-65.

T. Tokutomi, T. Kiyokawa, C. Shibayama, H. Harada, A. Ohashi (2006), Effect of inorganic carbon on nitrite accumulation in an aerobic granule reactor., Water Sci. & Tech., Vol.53, No.12, pp285-294.

T. Kosaka, T. Uchiyama, S. Ishii, M. Enoki, H. Imachi, Y. Kamagata, A. Ohashi, H. Harada, H. Ikenaga and K. Watanabe (2006), Reconstruction and Regulation of the Central Catabolic Pathway in the Thermophilic Propionate-Oxidizing Syntroph Pelotomaculum thermopropionicum, J. Bacteriol., vol.188, No.1, pp202-210

Qiu, Y.-L. Y. Sekiguchi, S. Hanada, H. Imachi, I-C. Tseng, S.-S. Cheng, A. Ohashi, H. Harada and Y. Kamagata (2006) Pelotomaculum terephthalicum sp. nov. and Pelotomaculum isophthalicum sp. nov.: two anaerobic bacteria that degrade phthalate isomers in syntrophic association with hydrogenotrophic methanogens, Archives of Microbiology

高橋優信、山口隆司、角野晴彦、荒木信夫、山崎慎一、長野晃弘、珠坪一晃、原田秀樹 (2006)、硫黄サイクル活性型UASB/DHSシステムによる低温低濃度有機性排水 (不凍液排水)処理技術の開発、環境工学論文集、Vol. 43, pp.353-358

阿部憲一、井町寛之、大橋晶良、原田秀樹、徳富孝明 (2006)、スポンジ – エアリフト型リアクターによる亜硝酸型硝化に及ぼすアンモニア濃度と温度の影響、環境工学論文集、Vol. 43, pp.343-351

川上周司、久保田健吾、井町寛之、大橋晶良、原田秀樹 (2006)、Two-pass TSA-FISH法によるメタン生成古細菌のmcr遺伝子の検出、環境工学論文集、Vol. 43, pp.143-148

角野晴彦、室田龍一、大橋晶良、原田秀樹、珠坪一晃 (2006)、嫌気性懸垂型スポンジろ床 (AnDHSリアクター)による低濃度有機性排水のメタン発酵処理、環境工学論文集、Vol. 43, pp.23-29

山田真義、片平智仁、山内正仁、大橋晶良、原田秀樹 (2006)、焼酎蒸留粕廃液を対象とした高温多段型UASBリアクターによるアルカリ剤削減オンサイト実証実験、環境工学論文集、Vol. 43, pp.7-14

酒井早苗、井町寛之、関口勇地、大橋晶良、原田秀樹、鎌形洋一 (2006)、嫌気性共生培養系を利用した未培養系統分類群に属するメタン生成古細菌の分離・培養、水環境学会誌、Vol. 29, No.7, pp.389-397

中村一彦、大橋晶良、井町寛之、原田秀樹 (2006)、UASBとDHSリアクターによる嫌気・好気処理システムの染色実廃水への適用、水環境学会誌、Vol. 29, No.10, pp.613-620

山崎慎一、山口隆史、荒木信夫、角野晴彦、原田秀樹 (2006)、2槽式UASB-接触酸化処理システムにおける有機物と窒素の除去特性、土木学会論文集、Vol. 62, No.1, pp.87-94

佐藤伸幸、大久保努、小野寺崇、大橋晶良、原田秀樹 (2006)、インドで運転されている各種下水処理プロセスの費用対効果に関する評価、環境システム研究論文集、Vol. 34, pp.575-583

<総説・解説・著書 >

井町寛之、酒井早苗 (2006)、難培養性メタン生成古細菌Rice Cluster Iの分離と特性土と微生物、Vol.60, No. 2, pp. 85-89.

井町寛之、関口勇地、大橋晶良、原田秀樹 (2006)、メタン発酵汚泥からの難培養性微生物の分離 (第2章第2節) 複合微生物系の産業利用と新産業創出 (シーエムシー出版), pp. 73-79.

カテゴリー: 研究業績 パーマリンク